Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures

Article Abstract:

A study was conducted on cloning and introducing the ubiE gene of Geobacillus stearothemophilus V, with its own promoter, into Escherichia coli. It was observed that the growth of cells in selenite- or selenate-amended media gave rise to the evolution of volatile selenium compounds, as a result of the ubiE gene in E. coli.

All Other Basic Inorganic Chemical Manufacturing, Industrial inorganic chemicals, not elsewhere classified, Selenium Compounds, Genetic aspects, Escherichia coli, Properties

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A group II intron-type open reading frame from the thermophile bacillus (Geobacillus) stearothermophilus encodes a heat-stable reverse transcriptase

Article Abstract:

A gene-designated trt is discovered from the genome of the thermophilic bacterium Bacillus (Geobacillus) stearothermophilus strain 10. The conclusion states that the trt protein is a heat-stable protein with RT activity and can reverse transcribe RNA at temperatures as high as 75 degree Celcius.

In-Vitro Diagnostic Substance Manufacturing, Diagnostic substances, Reverse Transcriptase

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Highly stable L-Lysine 6-Dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization gene cloning and sequencing, and expression

Article Abstract:

L-Lysine dehydrogenases catalyze the oxidative deamination of L-Lysine in the presence of NAD and so far two types of dehydrogenases were discovered. Thermophile and hyperthermophilic microorganisms were screened for a more stable form of L-Lysine dehydrogenase and results are discussed.

Influence

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