Extended screening by PCR for seven cry-group genes from field-collected strains of Bacillus thuringiensis

Article Abstract:

A research was conducted on the application of an extended multiplex polymerase chain reaction method created to determine and classify Bacillus thuringiensis strains containing crystal protein genes toxic to species of Coleoptera, Lepidoptera, and Diptera. Several reference strains were used to determine the reliability of primers while DNA templates were prepared from cultures in Luria-Bertani medium. Results showed that the DNA strains did not amplify the universal primers.

Usage, DNA, Bacillus thuringiensis, Polymerase chain reaction, Proteins

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Babesia spp. identified by PCR in ticks collected from domestic and wild ruminants in southern Switzerland

Article Abstract:

A survey of the occurrences of ruminant Babesia spp. and their tick vectors in Switzerland was conducted when a cattle herd in Switzerland was affected by concurrent infections. PCR analyses of 700 ticks revealed the presence of Babesia divergens, Babesia sp. genotype EU1 and B. major while identification of B. divergens and B. major in ticks collected from wild ruminants raised doubts on the postulation of strict host specificity of these bovine Babesia species.

Switzerland, Physiological aspects, Ticks

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Escherichia coli in settled-dust and air samples collected in residential environments in Mexico City

Article Abstract:

A study was conducted on Escherichia coli isolated from settled-dust and air samples in Mexico City, Mexico, residential areas. Antibiotic multiresistance was observed in 21% of the 89 Escherechia coli serotypes collected. Plasmid populations detected were also higher in antibiotic-resistant strains. Results reveal that the epidemological influence of environmental strain-produced intestinal infections are more serious than previously reported.

Health aspects, Escherichia coli, Mexico City, Mexico, Gastrointestinal diseases

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