Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins

Article Abstract:

A study was conducted to analyze mutS/hexA homologs from Saccharomyces cerevisiae as mismatch repair proteins. Amplification and cloning of these homologs was done through degenerate oligonucleotide primers based on E coli MutS protein regions and its homologs in polymerase chain reaction. A pair of DNA sequences were then amplified and used to clone genes from a yeast genomic library. Results show that the MutS homolog genes functioned in mitochondrial and nuclear DNA repair.

Recombinant proteins

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Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions

Article Abstract:

A study was conducted to evaluate MSH1 and MSH2 genes of Saccharomyces cerevisiae. These genes are assumed to encode proteins homologous to Escherichia coli MutS and Streptoccocus pneumoniae proteins and relative homologs. Disrupted MSH1 genes generate a rapidly developing phenotype. Results show that MSH1 functions in mitochondrial DNA repair or stability while MSH2 functions in 4-bp insertion/deletion mismatched repairs.

Research, Insertion elements, DNA, DNA insertion elements

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