Chlamydomonas: the cell and its genomes

Article Abstract:

The unicellular eukaryote Chlamydomonas reinhardtii features flagella and a chloroplast. Studies on Chlamydomonas are important in determining the protein components of the basal body and determining their functions in organelles. Several methods have been developed to duplicate Chlamydomonas genes wherein mutations generate novel and interesting phenotypes. Three of the most popular methods are insertional mutagenesis using plasmids, cloning by complementation and positional cloning.

Analysis, Eukaryotic cells, Cells (Biology), Eukaryotes

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Isolation of the Chlamydomonas regulatory gene NIT2 by transposon tagging

Article Abstract:

Researchers isolated the NIT2 gene of Chlamydomonas reinhardtii, employing a transposon tagging plan, to understand more about the role of the NIT2 gene product. The NIT2 gene encodes a 6-kb transcript that was not found in cells developed in the presence of ammonium. The repression of the NIT2 gene may mediate metabolite repression of the nitrate assimilation passage in Chlamydomonas.

Genetic aspects

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