Genomic organization of the entomopathogenic bacterium Bacillus thuringiensis subsp. berliner 1715

Article Abstract:

The physical and genetic mapping of Bacillus (B) thuringiensis subsp. berliner 1715 chromosome reveals 24 restriction enzyme sites and 28 probes. The genes are nonrandomly located on the 5.7 Mb chromosome. A comparison of the genetic maps of B. thuringiensis and B. cereus shows plasmid-encoded genes on the more variable half of the chromosome and rRNA genes and origin of replication on the other conserved half. The cryIA or Tn4430 probes' hybridization indicates large extrachromosomal DNA elements which show genome size to be at least 6.9 Mb.

Chromosome mapping

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Agrobacterium tumefacies possesses a fourth flagellin gene located in a large gene cluster concerned with flagellar structure, assembly and motility

Article Abstract:

A study was conducted to analyze a fourth flagellin gene in a 21850 bp region of the Agrobacterium tumefaciens C58C1 chromosome supporting at least 20 genes concerned with flagellar structure, assembly and function. Three flagellin genes were positioned in a tandem array at the right end. Results indicated that the flagellins contribute to flagellar structure. In addition, FlA has an anomolous electrophoretic mobility that ma be due to glycosylation.

Chromosomes, Agrobacterium tumefaciens

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Regulatory sequences of two flagellin genes in Bacillus thuringiensis subsp. alesti

Article Abstract:

An analysis of the transcription of two flagellin genes in Bacillus thuringiensis (Bt) 5 susp. alesti shows transcription from vegetative promoters during early- to mid-exponential growth phase. Several potential virulence factors were identified in Bt. Among them are phosphatidylcholine-degrading and phosphatidylinositol-specific C-type phospholipases, the beta-exotoxin thuringiensin, the immune inhibitor A and flagella.

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