Genotyping of human and porcine Yersinia enterocolitica, Yersinia intermedia, and Yersinia bercovieri strains from Switzerland by amplified fragment length polymorphism analysis

Article Abstract:

Amplified fragment length polymorphism (AFLP), a whole-genome fingerprinting method for subtyping bacterial isolates 231strains of Yersinia enterocolitica was used to analyze 25 strains of Y. intermedia and 10 strains of Y. bercovieri from human and porcine sources. AFLP typing distinguished the different Yersinia species examined and the AFLP technique is a valuable genotypic method for identification and typing of Y. enterocolitica and other Yersinia spp.

Analysis, Genotype, Genotypes

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Application of fluorescent amplified fragment length polymorphism for comparison of human and animal isolates of Yersinia enterocolitica

Article Abstract:

An amplified fragment length polymorphism (AFLP) method, developed to genotype Yersinia enterocolitica, is used to investigate 70 representative strains isolated from humans, pigs, sheep, and cattle in the UK. Results suggest that some strains causing human disease do not come from veterinary sources identifiable at this time

Genetic research

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Improved system for construction and analysis of single-copy beta-galactosidase operon fusions in Yersinia enterocolitica

Article Abstract:

A significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica is reported. A simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous beta-galactosidase activity is presented.

Microbiological chemistry, Microbial genetics, Beta galactosidases

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