Glucose-induced secretion of Trichoderma reesei xylanases

Article Abstract:

Gel electrophoresis analysis of Trichoderma reesei grown on glucose shows that recombinant strains carrying XYN 1 or XYN 2 structural genes are capable of producing the two xylanase isoenzymes, XYN 1 and XYN 2. The total xylanase productivity on xylan is three times that on glucose. However, the specific activities of the two xylanases increase on glucose because of the lesser amount of proteins produced. Electron microscopy and immunogold labelling have detected XYN 2 in the endoplasmic reticulum, Golgi-like vesicles, secretory vesicles, vacuoles, and cell walls.

Usage, Gel electrophoresis, Electron microscopy, Glucose

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Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.)

Article Abstract:

The in vivo and in vitro pathogenic actions of whole cells and extracellular products of Vibrio alginolyticus for cultured gilt-head sea bream were studied. The strains were able to fasten to the skin, gills and intestinal mucus of sea bream and to cultured cells of a chinook salmon embryo cell line. The extracellular products were observed to destroy sea bream mucus and tissues. V. alginolyticus was cytotoxic for fish cell lines and deadly for sea bream.

Diseases, Pathogenic microorganisms, Vibrio, Marine fishes

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