High-pressure-mediated survival of Clostridium botulinum and Bacillus amyloliquefaciens endospores at high temperature

Article Abstract:

The effects of combined pressure and temperature on Clostridium botulinum TMW 2.357 and B. amyloliquefaciens TMW 2.479, which are the most pressure-resistant spores, are investigated. The tailing and pressure-temperature combinations stabilizing bacterial endospores vary from strain to strain and hence food safety is necessary for studies demonstrating inactivation or nongrowth of C. botulinum with realistic contamination rates in the respective pressurized food and equipment.

Spores (Bacteria), Bacterial spores, Clostridium botulinum

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Expression and secretion of Bacillus amyloliquefaciens alpha-amylase by using ther yeast pheromone alpha-factor promoter and leader sequence in Saccharomyces cerevisiae

Article Abstract:

The construction of two yeast integrating shuttle vectors maintaining the pheromone alpha-factor prepro region in two different forms is discussed. Experiments involve gene cloning, transformation and integration of plasmid vectors into yeast strains and the analysis of the secretions of the amylase genes. The transcription of the amylase were late in the growth cycle compared to other yeast products.

Analysis, Saccharomyces, Amylases

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DegU and YczE positively regulate the synthesis of bacillomycin D by Bacillus amyloliquefaciens strain FZB42

Article Abstract:

A genetic tool kit is developed for Bacillus amyloliquefaciens strain FZB42 (FZB42) to decipher the complex regulatory network that has controlled the synthesis of bacillomycin D operon (dmy). The global regulators DegU and ComA are needed for the full transcriptional activation of dmy and DegU and YczE have posttranscriptionally regulated the expression of bacillomycin D.

Physiological aspects, Genetic regulation, Genetic transcription, Transcription (Genetics), Microbial pesticides

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