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Homofermentive lactate production cannot sustain anaerobic growth of engineered saccharomyces cerevisiae: Possible consequence of energy-dependent lactate export

Article Abstract:

Saccharomyces cerevisiae is engineered to homofermentative lactate-producing yeast through deletion of the three genes encoding pyruvate decarboxylase-negative S.cerevisiae strains, the engineered strain required small amounts of acetate for the synthesis of cytosolic acetyl-coenzyme. The conclusion states that the absence of net ATP production is probably due to a metabolic energy requirement for lactate export.

Author: Porro, Danilo, Dijken, Johannes P. van, Pronk, Jack T., Winkler, Aaron A., Maris, Antonius J. A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Usage, Risk factors, Microbiology, Lactates

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Physiological characterization of the ARO10-dependent, broad-substrate-specificity 2-oxo acid decarboxylase activity of Saccharomyces cerevisiae

Article Abstract:

A study was conducted to analyze the substrate specificity of the ARO10-dependant decarboxylase activity in Saccharomyces cerevisiae, and its impact on the production of fusel alcohols and acids. The studies conducted indicate the involvement of post-transcriptional regulation and a second protein in the ARO10-dependent, broad-substrate-specificity decarboxylse activity.

Author: Dickinson, J. Richard, Pronk, Jack T., Luttik, Marijke A.H., Vuralhan, Zeynep, Morais, Marcos A., Siew Leng Tai, Boer, Viktor M., Schipper, Dick, Almering, Marinka J.H., Kotter, Peter, Daran, Jean-Marc
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Genetic aspects, Decarboxylases

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Subjects list: Research, Brewer's yeast, Saccharomyces cerevisiae
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