Identification of Aspergillus brlA response elements (BREs) by genetic selection in yeast

Article Abstract:

The role of brlA gene in transcriptional activation and in the identification of sites for BrlA polypeptide interaction was evaluated from a minimal yeast promoter fused with upstream sequences of a developmentally regulated Aspergillus nidulans rod A gene. The DNA fragment from the promoter region displayed mediation of brlA-dependent activation. Two other DNA fragments which responded to brlA and identified sites for BrlA polypeptide interaction were labelled as brlA response elements (BREs). The BRE-containing DNA fragments affected the developmental regulation of Aspergillus.

Genetic aspects, Aspergillus, Identification and classification, Genetic regulation, Genetic transcription, Transcription (Genetics), Genetic code

---

On the consistency of a physical mapping method to reconstruct a chromosome in vitro

Article Abstract:

Previous studies related to genetic mapping have failed to evaluate the statistical consistency of physical mapping methods in reconstructing chromosomes. Based on the assumption that a staircase of DNA fragments exists within large randomly generated recombinant DNA libraries, a model of physical mapping by binary fingerprinting of DNA fragments was developed. Through the model, and on the assumption that an epi-convergence of a physical mapping method exists, it can be established that such mapping techniques possess strong statistical consistency.

Evaluation

---

The use of simulated annealing in chromosome reconstruction experiments based on binary scoring

Article Abstract:

Simulated annealing, a method of combinatorial optimization, can be utilized to draw up the position of clones along a chromosome. The method assigns binary digital numbers to each clone depending on the existence of particular sequences in the clone as determined by its hybridization with synthetic oligonucleotide probes. The similarity of digital assignment or signature of each clone determines the overlap between clones which can, in turn, be used to map the chromosome.

Methods

Similar abstracts:

• Abstracts: Identification of cryptic rearrangements in patients with 18q- deletion syndrome. Evidence for a novel osteosarcoma tumor-suppressor gene in the chromosome 18 region genetically linked with Paget disease of bone
• Abstracts: Expression of an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae. Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus
• Abstracts: Distribution of the transposable element mariner in anopheline mosquitoes. Fluorescence in situ hybridization analysis of hobo, mdg1 and Dm412 transposable elements reveals genomic instability following the Drosophila melanogaster genome sequencing
• Abstracts: Linkage analysis of endogenous viral element 1, blue eggshell, and pea comb loci in chickens. Expressed sequence tags for the chicken genome from a normalized 10-day-old white leghorn whole embryo cDNA library: 1. DNA sequence characterization and linkage analysis
• Abstracts: Preparation of encapsulated microbial cells for environmental applications. Microbial diversity and its relationship to planetary protection

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.