Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor

Article Abstract:

The microbial diversity of sludge from a deteriorated enhanced biological phosphorus removal reactor has been studied through denaturing gradient gel electrophoresis (DGGE). The reactor was treated with acetate and high levels of phosphate but failed to eliminate the phosphate in the effluent with limited phosphorus activity. DGGE was used in studying the 16S ribosomal DNA of the bacterial community. Results indicated the presence of the alpha subclass of Proteobacteria, the Legionella group of the gamma subclass of Proteobacteria and a novel group of the latter with no known association to any previously reported species.

Sewage, Microbial populations, Sewage treatment

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Construction and behavior of biologically contained bacteria for environmental applications in bioremediation

Article Abstract:

A recombinant biological containment system may be used to control the growth of Pseudomonas putida in response to the presence or absence of the alkylbenzoates that it degrades. In the absence of alkylbenzoates, a regulatory cascade drives expression from the Plac promoter fused to the gef gene, whose product is a killing protein. Contained strains were genetically stable and survived well as long as alkylbenzoates were present.

Analysis, Pseudomonas putida, Microbiological research

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Dual system to reinforce biological containment of recombinant bacteria designed for rhizoremediation

Article Abstract:

Researchers describe a biological containment system for recombinant strains of Pseudomonas putida that are used in bioremediation. These systems contain a killing gene, usually a porin-coding gene, that is activated when the pollutant is eliminated. This destroys the recombinant bacteria, making it less likely that they will remain in the environment.

Microbial genetic engineering

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