Identification of essential components of the S. cerevisiae kinetochore

Article Abstract:

A study to identify essential components of the Saccharomyces cerevisiae kinetochore is presented. An in vivo assay that detects the relaxation of the transcription block formed at the centromeres and another assay that detects an increase in the mitotic stability of a dicentric test chromosome were used to identify CTF13 and CTF14, two genes which encode kinetochore component proteins. Genetic and biochemical studies showed that the 478-amino acid protein product of CTF13 showed that CTF13 is essential for normal functioning of kinetochores, and thus for normal chromosomal segregation.

Composition, Saccharomyces, Cell division, Cell organelles, Organelles

---

CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition

Article Abstract:

Antibodies and cDNAs of human homologs of S. cerevisiae tetratrico peptide repeat (TPR)-containing sequences CDC27 and CDC16 have been localized in the nucleus, centrosome and the mitotic spindle. Antibodies against the human proteins CDC27Hs and CDC16Hs have been found to arrest the cell cycle during metaphase without affecting spindle structure. These proteins have been postulated to be involved in the control of the transition from metaphase to anaphase during mitosis.

Analysis, DNA, Nucleoproteins, Mitosis, Centrosomes, Centrosome

---

Regulating the yeast kinetochore by ubiquitin-dependent degradation and Skp1p-mediated phophorylation

Article Abstract:

The importance of protein subunit p23(super Skp1) in the Cbf3p complex function was investigated through analysis of coexpressing cells in the baculovirus expression system. Results have shown that p23(super Skp1) mediated the phosphorylation of p58. In addition, the protein subunit was also responsible for activation of the DNA binding through ubiquitin-conjugating complex.

Yeast, Yeast (Food product), Phosphorylation, Proteolysis, Ubiquitin

Similar abstracts:

• Abstracts: Identification of two proline transport systems in Staphylococcus aureus andtheir possible roles in osmoregulation
• Abstracts: Identification distinct communities of sulfate-reducing bacteria in oil fields by reverse sample genome probing
• Abstracts: Modification of the Fe protein of nitrogenase in natural populations of Trichodesmium thiebautii. Determination of nitrogen-fixing phylotypes in Lyngbya sp. and Microcoleus chthonoplastes cyanobacterial mats from Guerrero Negro, Baja California, Mexico
• Abstracts: Nucleosome displacement in transcription. A subset of TAF(sub II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation
• Abstracts: The adaptive significance of eggshell removal by nesting birds: testing the egg-capping hypothesis. Factors affecting renesting in American Coots

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.