Cadmium uptake and subcellular compartmentation in the ectomycorrhizal fungus Paxillus involutus

Article Abstract:

Subcellular compartmentation and cadmium uptake in the ectomycorrhizal fungus Paxillus involutus were studied using radiotracer flux analysis. It was found that ectomycorrhizal P. involutus has ability to take up and further accumulate Cd in different compartments. This is a first step in work toward understanding metal-tolerance mechanisms in mycorrhizal fungi.

France, Cytochemistry, Cells, Cell membranes, Cell permeability, Molds (Fungi), Cadmium

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Identification of genes differentially expressed in extraradical mycelium and ectomycorrhizal roots during Paxillus involutus-Betula pendula ectomycorrhizal symbiosis

Article Abstract:

The use of a two-dimensional peat microcosm that allows the formation of an intact mycorrhizal network from the Paxillus involutus-Betula pendula association with a cDNA array approach is investigated. It is concluded that in ectinycirrhizal root tips (ECM), a gene coding for a putative phoshatidylserine decarbozylase (Psd) was up-regulated 4.1-and 6.2-fold in extraradical mycelium (EM).

Science & research, Analysis, Genetic aspects, DNA microarrays, Mycelium, Mycelia

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Kinetics, energetics and specificity of a general amino acid transporter from the ectomycorrhizal fungus Paxillus involutus

Article Abstract:

The amino acid transport system in the ectomycorrhizal fungus Paxillus involutus shows characteristics of active transport. The uptake rate of glutamate, glutamine, aspartate and alanine amino acids increase with increasing substrate concentration without saturation. The 4 amino acid uptake systems have similar V(sub max) and K(sub m) values. Amino acid transport inhibition occurs in the presence of carbonyl cyanide m-chlorophenylhydrazone, dinitrophenol and NaN(sub 3). There is a lack of specificity in the transport system due to broad substrate recognition.

Biological transport, Active, Active biological transport

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