Identification of root rot fungi in nursery seedlings by nested multiplex PCR

Article Abstract:

A nested multiplex PCR approach allows the direct determination of root rot fungi in infected seedlings by amplifying two different species specific PCR fragments with individual primer combinations from genomic DNA. The first amplification with fungus specified primers increases the population of fungal internal transcribed tracer (ITS). This increases the ratio of fungal:host ITS molecules. In the second round of amplification, the ITS molecules acted as a template for the species-specific primers. The problems with this method are the danger of carry over contamination and high costs.

Microbiological assay, Fungi, Pathogenic, Pathogenic fungi

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A plant growth-promoting bacterium that decreases nickel toxicity in seedlings

Article Abstract:

The plant growth-promoting bacterium, Kluyvera ascorbata SUD165, was isolated from soil collected near Sudbury, Ontario, Canada. The bacterium contained high levels of heavy metals and was resistant to the toxic effects of nickel, tin and zinc. Protection by the bacterium against nickel toxicity was evident in pot experiments with canola and tomato seeds. Findings suggested that the growth-promoting effect in the presence of nickel was not attributable to the reduction of nickel uptake by seedlings.

Health aspects, Environmental aspects, Soil microbiology, Nickel, Growth (Plants), Plant growth, Heavy metals, Heavy metal poisoning, Sudbury, Ontario

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Multiplex polymerase chain reaction for detection and differentiation of themicrobial insecticide Bacillus thuringiensis

Article Abstract:

The utilization of a multiplex polymerase chain reaction (PCR) for the rapid identification of Bacillus thuringiensis strains is discussed. A PCR product characteristic profile was generated for each strain of B. thuringiensis subsp. kurtski. The electrophoretic pattern of the PCR products were the basis of the differentiation among these strains. Theutility of the strain detection method for commercial and field application wasdemonstrated.

Bacillus thuringiensis

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