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Molecular characterization of Pseudomonas putida KT2440 rpoH gene regulation

Article Abstract:

Pseudomonas putida KT2440 rpoH gene regulation is discussed based on molecular characterization. The gene encodes the heat shock sigma factor sigma (super.32). An inverted repeat sequence 20 bp downstream of the rpoH stop codon acts as a terminator in vivo in P. putida growing at 18 degrees C to 42 degrees C.

Author: Ramos, Juan L., Manzanera, Maximino, Aranda-Olmedo, Isabel, Marques, Silvia
Publisher: Society for General Microbiology
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
Spain, Statistical Data Included, Bacterial growth, Microbiological chemistry

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Identification of the heat-shock sigma factor RpoH and a second RpoH-like protein in Sinorhizobium meliloti

Article Abstract:

Results show that two DNA segments from Sinorhizobium meliloti exhibit sequence similarity to rpoH genes, encoding the sigma(sup)32 RpoH heat -shock transcription factor. Of the two genes, rpoH1 and rpoH2, the former upon mutation impairs growth and affect nitrogen fixation during symbiosis with alfalfa.

Author: Oke, Valerie, Rushing, Brenda G., Fisher, Emily J., Moghadam-Tabrizi, Mohamad, Long, Sharon R.
Publisher: Society for General Microbiology
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
United States, Analysis, Gene expression, Cell culture, Tissue culture, Rhizobium meliloti

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Survival in soil of different toluene-degrading Pseudomonas strains after solvent shock

Article Abstract:

The solvent tolerance of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina and their survival in soil after solvent shock are analyzed. The study revealed that the viability of P. putida DOT-T1 and F1 did not disappear upon heptane and propylbenzene shock. It was severely affected, however, by toluene shock even if both strains recovered and colonized polluted niches. This recovery was dependent upon soil humidity and the incubation temperature.

Author: Huertas, Maria-Jose, Duque, Estrella, Ramos, Juan L., Marques, Silvia
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
Environmental aspects, Toluene, Soil temperature

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Subjects list: Research, Physiological aspects, Genetic aspects, Heat shock proteins, Pseudomonas putida
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