Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei

Article Abstract:

The RutC-30 strain of the filamentous fungus Trichoderma reesei, engineered to overexpress T. harzianum chromosomal endochitinase gene ThEn-42, produces a large amount of active endochitinase. ThEn-42 is controlled by the cellulase gene cbh1 promoter. T. reesei produces almost 20 times the amount of enzyme produced by T. harzianum. Enzyme production increases in the presence of multiple copies of ThEn-42 and cbh1. T. reesei processes the enzyme's pro and prepro areas similar to T. harzianum. The endochitinase is degraded by an acid protease that is produced during the later growth stages in T. reesei.

Molds (Fungi)

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Enhanced expression of endochitinase in Trichoderma harzianum with the cbh1 promoter of Trichoderma reesei

Article Abstract:

The mycoparasitic fungus Trichoderma harzianum containing the T. reesei cellulase gene promoter cbh1 has greater endochitinase production and activity than the wild type fungus. T. harzianum grown on glucose or sucrose shows no extracellular endochitinase activity. This is probably due to a low endochitinase content as cbh1 is inactive under these conditions. The transformed cells overexpress endochitinase when grown in a cellulase-inducing culture medium or in the presence of sophorose. T. harzianum is a biological control for fungal plant pathogens as its endochitinase degrades fungal cell walls.

Analysis, Fungi, Pathogenic, Pathogenic fungi

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Interaction of ammonium, glucose, and chitin regulates the expression of cell wall-degrading enzymes in Trichoderma atroviride strain P1

Article Abstract:

Cell wall-degrading enzymes of Trichoderma atroviride strain P1 are regulated by other substances besides carbon catabolites and fungal cell wall components. This fungus occurs in soil and some strains have antifungal properties.

Soil fungi

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