Improved secretion of native human insulin-like growth factor 1 from gas1 mutant Saccharomyces cerevisiae cells

Article Abstract:

Researchers have been able to increase the secretion of human insulin-like growth hormone from yeast by fusing the hIGF-1 gene to the prepro-alpha-factor leader sequence and inactivating the GAS1 gene of the yeast. This hormone is of interest to the pharmaceutical industry.

Microbial biotechnology, Insulin-like growth factor 1, Insulin-like growth factor I

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Induction by hypoxia of heterologous-protein production with the K1PDC1 promoter in yeasts

Article Abstract:

The possibility of a practical application of the hypoxic regulation of the K1PDC1 promoter is investigated by cloning the whole promoter sequence upstream to several heterologous genes coding for proteins widely heterogeneous in their characteristics is presented. Based on the results it is proposed that the Zygosaccharomyces bailii yeast could be used as K1PDC1 promoter-based expression vectors to achieve heterologous production in a simple and cheap procedure.

Science & research, Genetic aspects, Gene expression, Yeast fungi, Yeasts (Fungi)

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Replacement of a metabolic pathway for large-scale production of lactic acid from engineered yeasts

Article Abstract:

The development of a homolactic Kluyveromyces lactis yeast strain, which displays high concentration and high productivity of lactic acid at a pH level below 5 to 5.5, the typical minimum pH range for lactate production by conventional microbial cells, is reported. Results represent the first example of a total replacement of a fermentation product mediated by metabolic engineering methods in yeast genera.

Microbial metabolism, Lactic acid, Yeast, Yeast (Food product)

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