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In situ and in vitro gene expression by Vibrio vulnificus during entry into, persistence within, resuscitation from the viable but nonculturable state

Article Abstract:

Clinical and environmental isolates of Vibrio vulnificus in estuarine waters was undertaken to monitor the expression of several genes during the VBNC state, and to study in situ gene expression of vibrio vulnificus during winter months due to the entrance of these cells into the viable but nonculturable (VBNC) state. The results were compared with the studies undertaken in vitro gene expression, which show that the loss of catalase activity is a direct result of katG repression, which accounts for the VBNC response of this pathogen.

Author: Oliver, James D., Smith, Ben
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Environmental aspects, Estuarine ecology, Estuarine ecosystems

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Evidence for an intermediate colony morphology of Vibrio vulnificus

Article Abstract:

Vibrio vulnificus causes both food-borne disease and wound infections and most V. vulnificus strains express capsular polysaccharide (CPS), which is required for the virulence of this organism. The wzb gene, which encodes a phosphotase required for CPS expression is proposed to be involved in the switch from opaque {Op, [CPS.sup.+]} colony morphology to a translucent {Tr, [CPS.sup.-]} colony morphology.

Author: Oliver, James D., Smith, Ben, Rosche, Thomas M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Polysaccharides, Structure, Chemical properties

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In situ gene expression by Vibrio vulnificus

Article Abstract:

A study was conducted on Vibrio vulnificus that possess two genes (wza and wzb), which code for a sugar transferase to observe under starvation-survival conditions. The findings suggest that while capsule synthesis continues in starved cells of Vibrio vulnificus, it is repressed in strains of the clinical genotype during entry into the viable but noncultureable state.

Author: Oliver, James D., Smith, Ben
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006

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Subjects list: Research, Genetic aspects, Gene expression, Vibrio
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