In situ chromosomal localization of rDNA sites in "Safed Musli"Chlorophytum Ker-Gawl and their physical measurement by fiber FISH

Article Abstract:

Fluorescence in situ hybridization (FISH) technique is applied on somatic chromosomes and extended DNA fibers in the medicinally important species of Chlorophytum to elucidate physical localization and measurement of the rDNA sites using two rRNA multigene families homologous to 45S and 5S rDNA. The two species of Chlorophytum such as C.borivillianum and C. comosum, both with 2n=28, reveal diversity for copy number and localization of rDNA sites.

Genetic research, Hybridization, Green algae

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Evolution by reticulation: European dogroses originated by multiple hybridization across the genus Rosa

Article Abstract:

Molecular evidence through analysis of nuclear ribosomal DNA data that dogroses are complex allopolyploids resulting from multiple hybridization events is reported. Findings reveal that the origin of pentaploid Caninae genome was originated from different members of nondogroses and the now extinct Protocaninae.

Roses, Allopolyploid, Allopolyploidy

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Experiments in DNA extraction and PCR amplification from bighorn sheep feces: The importance of DNA extraction method

Article Abstract:

A simple and general method to maximize reliability and efficiency of genotyping for such DNA extraction techniques and sample materials used is presented. The DNA extraction method probably has wide applicability to herbivores that produce pelleted feces where samples dry rapidly after deposition.

Genotype, Genotypes, Bighorn sheep

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