In situ identification of cyanobacteria with horseradish peroxidase-labeled, rRNA-targeted oligonucleotide probes

Article Abstract:

Cyanobacterial cells have been identified by using a whole-cell hybridization method that uses horseradish peroxidase (HRP)-labeled, rRNA-targeted oligonucleotides. The technique was used because fluorescent monolabeled oligonucleotides fail to overstain the autofluorescence of the target cells. The genotypic method has adequate sensitivity to detect cyanobacterial cells in the deeper layers of microbial mats. Its cell fixation, specificity and sensitivity were evaluated by using four oligonucleotides to detect four groups of bacteria.

Methods, Analysis, Nucleic acid probes, Microbial populations, Microbiological assay

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Effect of the nitrogen source on phycobiliprotein synthesis and cell reserves in a chromatically adapting filamentous cyanobacterium

Article Abstract:

Cell growth in cyanobacteria takes place with nitrate or ammonium as a fixed nitrogen source. In Calothrix sp. strain PCC 7601, nitrate and ammonium differ in their influences on the phycobiliprotein composition of the light-harvesting antennae known as psychobilisomes. These differences confirm common regulatory elements between acclimation to the spectral light quality and the nitrogen source.

Nitrogen-fixing microorganisms

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Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria

Article Abstract:

Results show that three products of internal transcribed spacer sequence are discernible between 16S-23S rRNA in Cyanobacterium Nostoc PCC 7120. Data indicate that the conserved and variable regions of these spacer sequences can serve as PCR primer targets as well as oligonucleotide probes in the detection and identification of cyanobacteria.

United States, France, Genetic aspects, Polymerase chain reaction, Genetic regulation, Ribosomal RNA

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