In vivo levels of chlorinated hydroquinones in a pentachlorophenol-degrading bacterium

Article Abstract:

The pentachlorophenol (PCP) monooxygenase and tetrachlorohydroquinone (TCHQ) dehydrogenase activities of Sphingomonas chlorophenolica were analyzed to determine the toxic levels of chlorinated hydroquinones in the cytoplasma. The pathway degradation of PCP in Sphingomonas chlorophenolica ATCC 39723 strain involved a monooxygenase and a TCHQ dehydrogenase that catalyzes the conversion of PCP to TCHQ. Furthermore, the toxicity of TCHQ on the cells was concentration-dependent and low levels of the compound did not induces adverse effects in the cells.

Microbiological synthesis, Soil microbiology, Pentachlorophenol

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Enzymic dehalogenation of 4-chlorobenzoyl coenzyme A in Acinetobacter sp. strain 4-CB1

Article Abstract:

High-performance liquid chromotography (HPLC) studies reveal that Acinetobacter sp. strain 4-CB1 degrades 4-chlorobenzoate by initially producing 4-chlorobenzoyl coenzyme A from 4-chlorobenzoate, ATP and CoA. This 4-chlorobenzoyl CoA is then dehalogenated and hydrolyzed to the finalproducts 4-hydroxybenzoate and CoA. An amended pathway for 4-chlorobenzoate degradation based on the results of this HPLC study is presented. The results show the importance of Coenzyme A in this process but its exact role is still unknown.

Acetylene, Neisseriaceae

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Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Anthrobacter sp. strain SU

Article Abstract:

The hydrolytic dehalogenation of 4-chlorobenzoate (4-CBA) isdiscussed. Anthrobacter sp. strains and TM1 (12) were used in this physiological and chemical study wherein dehalogenase genes were subjected to genetic analysis. These genes were cloned and expressed in Escherechia coli. The nucleotide sequence of the 4-CBA region was also determined and presented. Various degrees of homology were found between genes, proteins and amino acids.

Methods, Usage, Cloning, Amino acid sequence, Amino acid sequencing

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