Induction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F

Article Abstract:

Induction and transcription of the dextransucrase (DS) gene in Leuconostoc mesenteroides NRRL B-512F have been studied to find genetic evidence to help show whether or not the genes for DS and for sucrose-phosphorylase are under control of the same promoter. Isolation and characterization of mRNA have molecular information on the transcript. It was concluded that DSs from cells grown on various carbon sources come from the transcription of one gene. An activating phenomenon may be involved in the expression.

France, Mexico, Statistical Data Included, Genetic aspects, Fermentation, Oligosaccharides, Cellular signal transduction

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Leuconostoc mesenteroides B-1355 mutants producing alternansucrases exhibiting decreases in apparent molecular mass

Article Abstract:

All mutant strains of Leuconostoc mesenteroids B-1355 produce principal and minor alternansucrase bands, with decreased apparent molecular mass, on SDS-gels upon exposure to SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The mutant strains constitutive for glucosyltransferase synthesis on sugars apart from sucrose produce similar activity bands after growth in glucose and sucrose mediums. Deactivated dextransucrase is reactivated upon exposure to SDS-PAGE.

Microbial mutation, Sucrose

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Insoluble glucan formation by Leuconostoc mesenteroides B-1355

Article Abstract:

Experimental studies reveal that one of the three glucosyltransferases (GTFs) GTF-1, produced by Leuconostoc mesenteroides B-1355 is an unreported sucrase that synthesize water-insoluble dextran. Previously characterized GTF-2, an alternansucrase, is a cell-associated enzyme and GTF-2 accounts for only 10% of the total GTF activity in young, active culture supernatant fractions.

Microbial enzymes

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