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Induction of cellulase by gentiobiose and its sulfur-containing analog in Penicillium purpurogenum

Article Abstract:

The mechanism of induction of cellulase by gentiobiose and its sulfur containing analog, thiogentiobase, in mycelia of Penicillium purpurogenum P-26 was determined. In the presence of norijmycin which inhibited beta-glucosidase activity, gentiobase induced cellulase. However, without norijmycin in the induction mdeium, no inductive effect was observed which was attributed to hydrolytic action of beta-glucosidase on gentiobiose. Thiogentiobiose was a more effective because it induced cellulase even in the absence of norijmycin. In addition, it was not metabolized during the induction process.

Author: Tomita, Fusao, Kamagata, Yoichi, Kurasawa, Takashi, Yachi, Makoto, Suto, Manabu, Takao, Shoichi
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992

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Enigmatic gratuitous induction of the covalent flavoprotein vanillyl-alcohol oxidase in Penicillium simplicissimum

Article Abstract:

The growth of Penicillium simplicissimum on anisyl alcohol, veratryl alcohol or 4-(methoxymethyl)phenol causes the formation of the covalent flavoprotein, vannillyl-alcohol oxidase. The induction of the enzyme is highest during the growth phase, and is suppressed by the addition of isoeugenol to cultures containing veratryl alcohol. However, the formation of the enzyme is nonessential, as the degradation pathways of veratryl and anisyl alcohols have no requirement for vannillyl-alcohol oxidase. The enzyme is involved in the metabolism of 4-(methoxymethyl)phenol and p-cresol methylether.

Author: Fraaije, Marco W., Pikkemaat, Mariel, Van Berkel, Willem J.H.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Physiological aspects, Observations, Bacterial growth, Oxidases, Molds (Fungi)

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Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei

Article Abstract:

The basic characteristics of regulation of expression of the genes encoding the cellulases of the filamentous fungus Trichoderma reesei QM9414, the genes cbh1 and cbh2 encoding cellobiohydrolases and the genes egl1, egl2 and egl5 encoding endonuclease, were examined at the mRNA level. Findings revealed that distinct and clear-cut mechanisms of induction and glucose repression control cellulase expression in an actively growing fungus. However, derepression of cellulase expression is not accompanied by an addition of an inducer once glucose has been used up from the medium.

Author: Penttila, Merja E., Ilmen, Marja, Saloheimo, Anu, Onnela, Maija-Leena
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Genetic aspects, Fungi, Genetic regulation

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Subjects list: Research
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