Induction of the tod operon by trichloroethylene in Pseudomonas putida TVA8

Article Abstract:

A study was conducted to test whether trichloroethylene (TCE) influences the tod operon in the bioluminescent reporter Pseudomonas putida TVA8 using biochemical and molecular methods. The growing-cell assay was utilized to determine the bioluminescence response over time of TVA8 to different concentrations of TCE. Results indicated that specific bioluminescence from growing-cell assays can be utilized to determine enzyme activity on toluene-induced bioreactor and groundwater samples.

Research, Enzymes, Pseudomonas putida, Trichloroethylene

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NAH plasmid-mediated catabolism of anthracene and phenanthrene to naphthoic acids

Article Abstract:

The napthalene plasmid degradative enzyme system is involved in the degradation of higher-molecular-weight polycyclic aromatic hydrocarbons other than napthalene. The metabolism of anthracene and phenanthrene by plasmid pKA1 is confirmed, and by using mutant strain 5RL which contains the lux reporter gene transposon (Tn 4431) in the sal operon of pKAI, the role of the NAH upper pathway operon is demonstrated.

Usage, Plasmids, Metabolism

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A bioluminescent whole-cell reporter for detection of 2,4-dichlorophenoxyacetic acid and 2,4-dichlorophenol in soil

Article Abstract:

Research describes the construction of a reporter molecule for eliciting bioluminescent response from cells that receive it. Results show that when introduced on to Ralstonia eutropha JMP134 chromosome, the reporter produced bioluminescent response to herbicides.

United States, Genetic aspects, Biodegradation, Herbicides, Gene fusion, Bioluminescence

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