Isolation and characterization of SUA5, a novel gene required for normal growth in Saccharomyces cerevisiae
Article Abstract:
A new gene, sua5, necessary for normal growth of yeast was identified, isolated and characterized. The gene is located on chromosome VII with a related gene on chromosome XII. The locus suppresses an erroneous ATG codon in cyc1 gene's leader region. sua5 apparently codes for a 46,537 dalton protein that agrees well with the transcript analyses, further, a 44 kilodalton protein was identified using antiserum. Aberation in the sua5 gene lead to failure to utilize glycerol or lactate as carbon source indicating that the gene product is essential in normal glycolysis and respiratory functions.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Strand-specificity in the transformation of yeast with synthetic oligonucleotides
Article Abstract:
Transformation of Cyc1 mutant Saccharomyces cerevisiae with antisense and sense oligonucleotide was undertaken to analyze the transformation role of two genomic DNA strands. Sense oligonucleotides produced 20 times the number of transformation from antisense DNA sequence. The difference in the effects of the oligonucleotides was not dependent on sequence, mismatched nucleotides or length. It was also independent of the strain of the host in which the vector was introduced.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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cis- and trans-acting suppressors of a translation initiation defect at the cyc1 locus of Saccharomyces cerevisiae
Article Abstract:
Three classes of functional revertants of Saccharomyces cerevisiae's cyc1-362 mutant have been isolated and classified. These revertants help identify factors that act in cis or in trans to suppress the adverse effect of the open reading frame (uORF) in the cyc1 gene's leader region. The revertants can be used to develop suppressor mechanisms.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
User Contributions:
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