Isolation and characterization of acid-tolerant, thermophilic bacteria for effective fermentation of biomass-derived sugars to lactic acid

Article Abstract:

The isolation and physiological characterization of three new gram-positive bacterial isolates with growth optima that match those for fungal cellulases are described. The new Bacillus coagulans isolates can be used to reduce the cost of simultaneous saccharification and fermentation (SSF) by minimizing the amount of fungal cellulases, a significant cost component in the use of biomass as a renewable resource, for the production of fuels and chemicals.

Research and Development in the Physical, Engineering, and Life Sciences, Regulation and Administration of Communications, Electric, Gas, and Other Utilities, Pharmaceutical Preparation Manufacturing, All Other Basic Organic Chemical Manufacturing, Pharmaceutical preparations, Industrial Organic Chemicals, Carbohydrates, Lactic Acid, Biomass R&D, Energy R&D Programs-Biomass, Bacteria, Thermophilic, Chemical properties, Thermophiles, Biomass energy research

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Functional replacement of the Escherichia coli D-(-)-lactate dehydrogenase gene (ldhA) with the L-(+)-lactate dehydrogenase gene (ldhL) from Pediococcus acidilactici

Article Abstract:

Research describes the derivation of a Escherichia coli biocatalyst strain carrying five chromosomal deletions and a chromosomally integrated L-(+)-LDH gene from Pediococcus acidilactis. The transformed E. coli does not contain plasmids or antibiotic resistance genes but can produce optically pure L-(+)-lactic acid using glucose and xylose.

United States, Analysis, Microbiological synthesis, Chromosome deletion, Lactate dehydrogenase, Genetic transformation

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Construction of an Escherichia coli K-12 mutant for homoethanologenic fermentation of glucose or xylose without foreign genes

Article Abstract:

A novel approach relying on only native enzymes is used to develop a homoethanologenic alternative, Escherichia coli strain SE2378. Anaerobic ethanol production by the mutant is the result of a novel pathway that combines the activities of pyruvate dehydroxygenase with the fermentative alcohol dehydrogenase.

Gene mutations, Gene mutation, Escherichia coli, Pyruvate dehydrogenase complex

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