Isolation and characterization of microsatellite loci for use in population genetic analysis in the timber rattlesnake, Crotalus horridus

Article Abstract:

Microsatellite markers are useful in the population genetics analysis in the timber rattlesnake, Crotalus horridus. Primers developed to amplify six microsatellite loci are useful in screening 32 individuals from different geographical localities. The microsatellites are polymorphic and have two to nine alleles with heterozygote frequencies from 0.1 to 0.69, which vary with the geographical location of populations. The markers are useful in determining the relationships and genetic diversity between and within the populations of C. horridus.

Research, Genetic aspects, Biological diversity, Biodiversity, Rattlesnakes

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Proportion of the variation in genetic composition in backcrossing programs explained by genetic markers

Article Abstract:

Most of the variance of genetic composition for a randomly mating backcrossing population is described by three or more markers for each chromosome. A single marker in the middle of a chromosome is more efficient in explaining the variance than two markers at the ends. An extension of Hill's results is used to derive the degree of variance possible by genetic markers. The markers give an estimate of the mean proportion of the genome that belongs to the recurrent breed.

Analysis, Genomes, Variation (Biology)

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