A homokaryotic derivative of a Phanerochaete chrysosporium strain and its use in genomic analysis of repetitive elements

Article Abstract:

Researchers illustrate the use of cultures derived from a single basidiospore of Phanerochaete chrysosporium for genetic analysis of transposons. These haploid homokaryons contain a single nuclear type, unlike the two different nuclear types in dikaryons.

Genetic aspects, Fungi, Phytopathogenic, Phytopathogenic fungi, Transposons

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Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase

Article Abstract:

A protocol for identification and differentiation of allelic variants of the lignin peroxidase gene in Phanerochaete chrysosporium was developed and is presented. The method involves genomic DNA amplification by the polymerase chain reaction. The amplified genes are then probed with allele-specific oligonucleotides using the Southern blot technique. This protocol allows rapid identification of lignin peroxidase genes and alleles and is useful for classifying P. chrysosporium strains.

Analysis, Fungi, Peroxidase, Microbial genetics

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Isolation and purification of Pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation

Article Abstract:

Study done to identify and characterize the glucose-oxidizing activity of Phanerochaete chrysosporium grown on solid substrate is reported. 13C-nuclear magnetic resonance (NMR) and 13C-labeled glucose were used to unambiguously distinguish glucose 1-oxidase (GOX) from pyranose 2-oxidase (POX). High levels of pox transcript were found under carbon-starved ligninolytic conditions but not under nutrient sufficiency and thus, this regulation pattern supports the evidence that POX has a role in lignocellulose degradation.

Science & research, Genetic regulation, Microbiological research, Glucose, Dextrose

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