Kinetic studies and biochemical pathway analysis of anaerobic poly-(R)-3-hydroxybutyric acid synthesis in Escherichia coli

Article Abstract:

Poly-(R)-3-hydroxybutyric acid (PHB) was synthesized anaerobically in recombinant Escherichia coli. A theoretical biochemical network model was used to provide a rational basis to interpret the experimental results like the fermentation product secretion profiles and to study E. coli network capabilities under anaerobic conditions.

Biochemical genetics, Anaerobic bacteria

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Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays

Article Abstract:

Gene expression profiles were collected from Escherichia coli strains before and after exposure to organic solvents, and the six genes that showed higher gene expression were selected. Among these genes, glpC encoding the anaerobic glycerol-3-phosphate dehydrogenase subunitC remarkably increased the organic solvent tolerance.

Analysis, Gene expression, DNA microarrays

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Influence of growth phase on adhesion kinetics of Escherichia coli D21g

Article Abstract:

The influence of bacterial growth stage and the evolution of surface macromolecules on cell adhesion are examined by using a mutant of Escherichia coli K-12. Results suggest that the macromolecules responsible for this phenomenon are outer membrane-bound proteins and lipopolysaccharide-associated functional groups.

Bacterial growth, Cell adhesion

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