An explosive antisense RNA strategy for inhibition of a lactococcal bacteriophage

Article Abstract:

A method of rapidly producing antisense RNA against a Lactococcus lactis bacteriophage is described. The bacterium is used in the fermentation of dairy products, and a phage attack can substantially reduce its activity.

Usage, Lactobacillus, Antisense RNA

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Leaky Lactococcus cultures that externalize enzymes and antigens independently of culture lysis and secretion and export pathways

Article Abstract:

Research on Lactococcus lactis provides information useful for developing strains that can efficiently release relevant enzymes and proteins outside cells without known export and secretion signals, a significant fermentation innovation for the dairy industry. The research involved controlled expression of integrated phage holin and lysin cassettes.

United States, Statistical Data Included, Innovations, Cheese, Fermentation, Milk, Dairy bacteriology, Lysogeny

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Common elements regulating gene expression in temperate and lytic bacteriophages of Lactococcus species

Article Abstract:

Lytic and temperate bacteriophages of the P335 species of Lactococcus apparently have the same ancestors. This was found in an investigation of whether or not other lactococcal strains harbored sequences homologous to that of P(sub 566-888). Three lysogenic strains of the nine strains tested have homologous sequences. Mitomycin C induced the resident prophages in these strains and simultaneously induced the P(sub 566-888) promoter. According to DNA sequencing, the promoter elements in the three phages were identical to each other and to P(sub 566-888) from the lytic phage (phi)31.

Analysis, Genetic aspects, Genetic regulation, Gene expression

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