Mannan-degrading enzymes from Cellulomonas fimi

Article Abstract:

The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A and beta-mannosidase 2A, respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two other modules. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans. Man26A may be the only mannanase produced by the organism.

Genetic aspects, Escherichia coli, Biodegradation

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Analysis of molecular size distributions of cellulose molecules during hydrolysis of cellulose by recombinant Cellulomonas fimi beta-1,4-glucanases

Article Abstract:

The properties and activities of four cellulases of Cellulomonas fimi obtained from the purification of Escherichia coli were compared. Different types and amounts of soluble products resulted from the hydrolysis of phosphoric acid-swollen cellulose (PASC), although cellobiose was the major product obtained. The variability in the molecular size distributions data in both the PASC and the bacterial microcrystalline cellulose is influenced by their susceptibility to enzyme attacks.

Enzyme kinetics, Molecular weights

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Cellobiose dehydrogenase, an active agent in cellulose depolymerization

Article Abstract:

A study was conducted on the capacity of Phanerochaete chrysosporium-derived cellobiose dehydrogenase for Douglas fir kraft pulp modification. A decrease in pulp cellulose polymerization was observed when cellobiose and iron supplemented the cellobiosede dehydrogenase. Results reveal the generation of sugars arabinose and glucose. Results also indicate that cellobiose dehydrogenation stems cellulose polymerization reduction with minimal release of sugar.

Polymerization, Dehydrogenation

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