Development and characterization of diamondback moth resistance to transgenic broccoli expressing high levels of Cry1C

Article Abstract:

Results reveal that diamondback moth possessing a high level of resistance to Cry1C protoxin of Bacillus thuringiensis can sustain growth the entire life cycle on transgenic broccoli, also expressing high levels of Cry1C. Data point out that the Cry1C resistance is not related to any modification of the binding site.

United States, Australia, Spain, Statistical Data Included, Physiological aspects, Genetic aspects, Genetically modified plants, Moths, Broccoli, Insecticide resistance

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Mannose phosphate isomerase isoenzymes in Plutella xylostella support common genetic bases of resistance to Bacillus thuringiensis toxins in Lepidopteran species

Article Abstract:

Two mannose phosphate isomerase isoenzymes appear to confer resistance to the Bacillus thuringiensis Cry1A toxin in Plutella xylostella. This is the only insect known to be resistant to B. thuringiensis Cry1A toxin in the field, but others have demonstrated potential resistance in the laboratory.

Insect pests, Biological insect control

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Toxicity of Bacillus thuringiensis spore and crystal protein to resistant diamondback moth (Plutella xylostella)

Article Abstract:

The diamondback moth Plutella xylostella from Florida, USA, is resistant to Bacillus thuringiensis HD-1 spores and the CryIA crystal protein protoxins. The moth is sensitive to CryIB, CryIC and CryID protoxins while the CryIE and CryIIA proteins are nontoxic. HD-1 synergizes the activity of the proteins which are toxic and has no effect on those that are nontoxic. The toxicity is due to binding of the toxin to midgut and brush border membrane vesicles of the moth and reduced binding confers resistance to the toxin.

Analysis, Diamond-back moth, Diamondback moths

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