Measurement of marine picoplankton cell size by using a cooled, charge- coupled device camera with image-analyzed fluorescence microscopy

Article Abstract:

Application of a new technique in camera technology in the measurement of picoplankton cell sizeallows more accurate measurement as compared to other techniques. Charge- coupled device (CCD) camera with image-analyzed fluorescence microscopy performs better than video camera because of its high sensitivity and low noise Its high sensitivity enables detection of picoplanktons in their full size range based on the plankton fluorescence. Its high brightness resolution enables it to detect even very faint materials. Accurate measurement and counting of picoplankton can be obtained with this new method.

Measurement, Marine biology, Plankton populations

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Use of inorganic membrane filters (Anopore) for epifluorescence and scanningelectron microscopy of nanoplankton and picoplankton

Article Abstract:

The use of inorganic membrane filters (Anopore) to measure and identify nanoplankton and picoplankton on both epifluorescence and scanning electron microscopes was found to be more effective compared to other filters. Results of this study showed enhanced image clarity and resolution with epifluorescence microscopy due to the flatness and large porosity of the Anopore filters. No charging or darkening of phytoplankton cells was found using scanning electron microscope due to the aluminum oxide composition of thefilter which provided a stable background for microscopy.

Filters (Separation), Scanning electron microscopes

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Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures

Article Abstract:

Flow cytometry may be used for measuring 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) activity in natural assemblages of marine bacteria growing in dilution cultures. More CTC-positive cells were detected by flow cytometry than by visual epifluorescence microscopy. Cytometry may not detect all active cells because the dimmest fluorescing cells are not clearly separated from background noise. Addition of an artificial reducing agent, sodium dithionate, after CTC incubation and fixation resulted in a greater number of positive cells.

Analysis, Flow cytometry, Marine bacteria, Marine plankton, Chlorides

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