Mei-W68 in Drosophila melanogaster encodes a Spo11 homolog: evidence that the mechanism for initiating meiotic recombination is conserved

Article Abstract:

Meitotic recombination requires activity of several gene products in Drosophila melanogaster and Saccharomyces cerevisiae. Through use of a new genetic mapping method, mei-W68 has been cloned to give high-resolution mapping to a less-than-18-db region with a homolog of the S. cerevisiae spo11 gene. In the new mapping method, P elements are used to promote crossing-over at their insertion sites. Spo11 is proposed as the enzymatic activity that brings the double-strand breaks required to initiate meiotic recombination. Perhaps recombination in Drosophila is started by double-strand breaks. Evidence that the mechanism for initiating meiotic recombination is conserved exists.

Observations, DNA repair, Drosophila, Crossing over (Genetics)

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Rad52 associates with RPA and functions with Rad55 and Rad57 to assemble meiotic recombination complexes

Article Abstract:

Rad52, the Saccharomyces cerevisiae recombination protein, and RPA, the single-strand DNA-binding protein, combine into cytologically detectable subnuclear complexes in recombination in meiosis. Double-strand breaks (DSBs) are not adequate for the appearance of Rad51 foci, the detectable subnuclear complexes. They promote formation of RPA, Rad52 and Rad51 foci.

Analysis, Protein binding, DNA binding proteins

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