Mineralization of paraoxon and its use as a sole C and P source by a rationally designed catabolic pathway in Pseudomonas putida

Article Abstract:

Pseudomonas putida strain was engineered with two heterologous pathways to hydrolyze paraoxon and mineralize the hydrolysis products and use it as a sole carbon (C) and phosphorus (P) source. It was observed that there was no need for selection pressure to maintain the heterologous pathway, since the organism could utilize paraoxon as the sole C and P source and one of the intermediates in the pathways was toxic at high concentrations.

All Other Basic Organic Chemical Manufacturing, California, Industrial Organic Chemicals, Organophosphates, Organophosphorous Compounds, Genetic aspects, Pseudomonas putida, Hydrolysis, Organophosphorus compounds, Organic phosphorus compounds, Chemical properties

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Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity

Article Abstract:

A method to isolate terpene synthase clones from a population of bacterial clones expressing a DNA library is presented. Results conclude that the method discovered two genes yhfR and nudF whose protein products produced isopentenol by acting directly on the prenyl diphosphate precursors.

Usage, Growth, Bacillus subtilis, Cloning, Phenotype, Phenotypes, Company growth

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Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters

Article Abstract:

The directed evolution of AraC to build an arabinose-inducible system, which is more compatible with (isopropyl-[beta]-D-1-thiogalactopyranoside) induction of a lactose-inducible system is presented.

Escherichia coli, DNA-ligand interactions, DNA binding, Structure, Aldoses

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