Mini-chromosomes in Fusarium sporotrichioides are mosaics of dispersed repeats and unique sequences

Article Abstract:

A study of the degree of intraspecific karyotype variation in Fusarium sporotrichioides and the relationship between karyotype differences and toxin production patterns reveals the absence of any correlation between trichothecene pattern variations and electrophoretic karyotypic polymorphisms. The mini-chromosomes range exhibit interisolate karyotype differences. Summation of the NotI fragments reveals the average genomic size of F. sporotrichioides to be 20.5 Mb. The mini-chromosome that has common sequences with larger ones form a mosaic of dispersed repeats and unique sequences in F. sporotrichioides.

Analysis, Mosaicism, Chromosomes

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Identification of a CAP (adenylyl-cyclase-associated protein) homologous gene in Lentinus edodes and its functional complementation of yeast CAP mutants

Article Abstract:

Adenylyl-cyclase-associated proteins (CAP) are ubiquitous in nature being found in a variety of organisms from the fission yeast to humans. The gene coding for CAP in the mushroom Lentinus edodes has been identified and characterized. It has an open reading frame encoding a protein with 518 amino acid residues interrupted by eight introns. There is only a single copy of the gene and it is expressed uniformly. Complementation studies indicate that the carboxyl terminal end of CAP is conserved.

Shiitake

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Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats

Article Abstract:

The utility of the analysis of variable numbers of tandem repeats in the differentiation of species and strains belonging to the Mycobacterium tuberculosis complex is investigated. Eleven loci were identified and amplified in 48 strains. Twenty-two unique allele profiles were identified in 25 wild strains from the M. tuberculosis complex while five were identified in 23 substrains of the attenuated M. bovis.

Mycobacterium tuberculosis, Identification and classification, Mycobacterium, Mycobacterium bovis

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