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Mode of action of lactococcin B, a thiol-activated bacteriocin from Lactococcus lactis

Article Abstract:

Changes in the membrane potential, acidity gradient and amino acid uptake and efflux in the cells of Lactococcus lactis due to a purified lactococcin B (LcnB) bacteriocin is discussed. Measurements of membrane potential and intracellular acidity were determined by tetraphenylphosphonium-selective electrode and spectrofluorometry, respectively. Results indicate the presence of LcnB immune cells.

Author: Venema, K., Abee, T., Haandrikman, A. J., Leenhouts, K.J., Kok, J., Konings, W.N., Venema, G.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
Bacterial genetics, Lactic acid

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Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae

Article Abstract:

A novel NADP-dependent branched-chain alcohol dehydrogenase was isolated from Saccharomyces cerevisiae var uvarum developed in an anaerobic environment. Characterization of the alcohol dehydrogenase reveals that it has a monomeric quaternary structure, a 5.9 pI and a 37 kDa molecular mass. Results indicate that the enzyme may be used in making food flavorings during alcoholic fermentation.

Author: Abee, T., Rombouts, F.M., Iersel, M.F.M. van, Eppink, M.H.N., Berkel, W.J.H. van
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Enzymes, Saccharomyces, Alcohol dehydrogenase, Alcohol dehydrogenases

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Germination of Bacillus cereus spores is induced by germinants for differentiated caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine

Article Abstract:

The germination induction upon adhesion to caco-2 cells in 11 enterotoxigenic strains of Bacillus cereus was investigated. It was found that spores of the 11 strains of Bacillus cereus adhered with similar efficiencies to Caco-2 cells, Hep-2 cells did not trigger germination and spores of all strains displayed similar germination efficiencies.

Author: Abee, T., Wijnands, L.M., Dufrenne, J.B., van Leusden, F.M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
Analysis, Physiological aspects, Germination, Bacillus cereus

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Subjects list: Research, Genetic aspects
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