Molecular analysis of genetic differences between virulent and avirulent strains of Aeromonas hydrophila isolated from diseased fish

Article Abstract:

Results show that suppression subtractive hybridization technique enables identifying genetic differences and virulence genes in different strains of Aeromonas hydrophila. Data indicate that from among the virulent-specific genes, 22 DNA fragments are detected in virulent but not in avirulent strains.

Singapore, Statistical Data Included, Analysis, Identification and classification, Gene expression, Pathogenic microorganisms, Fish breeding

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Identification and characterization of putative virulence genes and gene clusters in Aeromonas hydrophila PPD134/91

Article Abstract:

A variety of putative virulence genes in Aeromonas hydrophila PPD134/9 were identified by analyzing both genomic subtraction and markers of genomic islands (GIs). It demonstrated that virulence in Aeromonas hydrophila is complex and involves multiple virulence factors which work in concerted nature of pathogenicity.

Science & research, Plasmids, Hybridization

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Internalization of Aeromonas hydrophila by fish epithelial cells can be inhibited with a tyrosine kinase inhibitor

Article Abstract:

The host-pathogen interaction and signal transduction pathway of the gram-negative bacteria Aeromonas hydrophila, which invades fish cells, were investigated utilizing carp epithelial cells and a virulent strain of the bacterium, PPD134/91. Findings indicated that tyrosine phosphorylation may have an important role in internalization. On the other hand, staurosporine, a protein kinase C inhibitor, and sodium orthovanadate, a protein tyrosine phosphatase inhibitor, accelerated internalization.

Microbiology, Epithelial cells, Protein tyrosine kinase, Protein-tyrosine kinase, Enzyme inhibitors

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