Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence

Article Abstract:

Sequencing of the Clostridium botulinum type B neurotoxin gene revealed that the neurotoxin was composed of 1,291 amino acids. The L chain was most homologous to that of tetanus toxin and the H chain to that of the type A neurotoxin. A high degree of amino acid conservation is present, most notably that of tryptophan in the H chain. The most divergent region is the extreme carboxy terminus for each toxin, reflecting differing specificities for neuronal acceptor sites.

Analysis, Nucleotide sequence, Base sequence, Botulinum toxin, Botulinum toxins, Amino acid sequence, Amino acid sequencing

---

A gene system for glucitol transport and metabolism in Clostridium beijerinckii NCIMB 8052

Article Abstract:

Clostridium beijerinckii possesses a gene system that codes for proteins necessary for glucitol transport and metabolism. These genes, designated as gut, were cloned on a chromosomal fragment from Escherichia coli and were found to occupy a region measuring four kilobases. There are five open reading frames in the cloned DNA fragment. The regulation of the expression of these genes is discussed.

Physiological aspects, Genetic aspects, Sorbitol

---

Analysis of the mechanism and regulation of lactose transport and metabolism in Clostridium acetobutylicum ATCC 824

Article Abstract:

The uptake and metabolism of lactose, the major sugar in industrial whey waste, by Clostridium acetobutylicum ATCC 824 is investigated. Lactose is taken up via a phosphoenolpyruvate-dependent phosphotransferase system and a pathway is proposed for the uptake and metabolism of lactose by an industrially important organism.

Science & research, Microbial metabolism, Lactose, Report

Similar abstracts:

• Abstracts: Epitope regions in the heavy chain of Clostridium botulinum type E neurotoxin recognized by monoclonal antibodies
• Abstracts: Alternative reading frames of the INK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest
• Abstracts: Transcription of the glnB and glnA genes in the photosynthetic bacterium Rhodospirillum rubrum. Characterization of the glnB gene product of Nostoc punctiforme strain ATCC 29133:glnB or the P(sub II) protein may be essential
• Abstracts: Splicing defects in the ataxia-telangiectasia gene, ATM: underlying mutations and consequences. Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome
• Abstracts: Engineering of plasmin-resistant forms of streptokinase and their production in Bacillus subtilis: streptokinase with longer functional half-life

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.