Molecular detection of Norwalk-like calciviruses in sewage

Article Abstract:

High loads of Norwalk-like viruses (NLVs) may be in sewage. That might justify more studies addressing the efficacy of NLV removal by sewage water treatment methods. NLV RNA was found by reverse transcriptase PCR (RT-PCR) in sewage water concentrates. Analysis of sequences of the RT-PCR products showed identical sequences in stools of patients and related sewage samples. In 6 of 11 samples not related to outbreaks, multiple NLV genotypes were seen.

Netherlands, Health aspects, Statistical Data Included, Causes of, Genetic aspects, Viruses, Sewage, Swimming, Drinking water, Enteritis, Molecular microbiology, Reverse transcriptase, Rotavirus infections, Viral diarrhea

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Analysis of nitrogen-fixing members of the [epsi] subclass of Proteobacteria in salt marsh sediments

Article Abstract:

The development of a molecular tool to target nitrogen-fixing bacteria with sequence similarities to members of the [epsi] subclass of Proteobacteria is reported. The specificity of the primer set is confirmed by the retrieval of nifH sequences of only putative members of the [epsi] subclass.

Environmental aspects, Identification and classification, Natural resources, Marine sediments, Nitrogen-fixing microorganisms

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Characterization and identification of numerically abundant culturable bacteria from the anoxic bulk soil of rice paddy microcosms

Article Abstract:

Research is conducted on the use of molecular and cultivation techniques to identify the number of the dominant bacteria in anoxic rice soil. Population sizes determined by liquid dilution cultures and isolated microorganisms from terminal dilution steps are examined.

International, Analysis, Soils, Bacteria, Rice, Cosmology, Microbiological research, Dilution, Dilution (Chemistry)

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