Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Article Abstract:

The cultivation and abundance of Roseburia- and Eubacterium rectale-related human gut bacteria in human feces was examined by comparing 16S rRNA sequences from 19 cultured human gut strains with 356 related sequences derived from clone libraries. Five out of six Roseburia- and E. rectale-related phylotypes or operational taxonomic units (OTUs) were defined by molecular diversity analyses through isolation under strict anaerobiosis, leading to the possibility of recovering a very high proportion of the total bacterial diversity present in the normal human gut as pure cultures.

Science & research, Observations, Molecular genetics, Hybridization, Nucleotide sequencing

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Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes

Article Abstract:

Research was conducted to examine the variations of bacterial populations in human feces measured by fluorescent in situ hybridization. Six 16S rRNA-targeted oligonucleotide probes were also conducted to quantify predominant groups of anerobic bacteria in the fecal samples. The probe combination detected at least two-thirds of the fecal flora while normal biological variations within the fecal populations of the volunteers were determined. Results suggest that the variations should be taken into account when assessing the effects of agents regulating the flora.

In situ hybridization, Nucleic acid probes, Fluorescence, Ribosomal RNA, Microbial populations

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Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups

Article Abstract:

Research shows that fecal samples from the formula-fed infants contain higher numbers of Coriobacterium group cells as compared to the breast-fed infants. Data also suggest with increasing age, the diversity of the Atopobium cluster species in the feces increases.

Netherlands, Statistical Data Included, Physiological aspects, Food and nutrition, Infants, DNA probes, Breast feeding

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