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Purification and molecular characterization of a tripeptidase (PepT) from Lactobacillus helveticus

Article Abstract:

Research describes purification and characterization of a tripeptidase in Lactobacillus along with data on cloning, DNA sequencing, and mRNA analysis of the gene encoding the enzyme.

Author: Savijoki, Kirsi, Palva, Airi
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
Proteases, Gene expression

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Metabolic engineering of Lactobacillus helveticus CNRZ32 for production of pure L-(+)-lactic acid

Article Abstract:

Research describes the usage of gene replacement technology to improve the production of lactic acid by Lactobacillus helveticus CNRZ32. Results show that in the genetically engineered strains lactic acid production was higher by 53 to 93% as compared to the wild-type strain.

Author: Palva, Airi, Kyla-Nikkila, Kari, Hujanen, Mervi, Leisola, Matti
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
Product enhancement, Genetic engineering, Lactic acid, Genetic regulation

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Molecular genetic characterization of the L-lactate dehydrogenase gene (ldhL) of Lactobacillus helveticus and biochemical characterization of the enzyme

Article Abstract:

The molecular genetic characterization of the L-lactate dehydrogenase gene (ldhL) of Lactobacillus helveticus and the biochemical characterization of the enzyme was examined by isolating it from a lambda library. Cloning, DNA sequencing and messenger RNA analysis of the ldhL gene from L. helveticus were also reported. Results show that the ldhL gene have the capacity to encode a protein of 323 amino acids.

Author: Savijoki, Kirsi, Palva, Airi
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Enzymes, Lactate dehydrogenase

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Subjects list: Statistical Data Included, Research, United States, Finland, Analysis, Physiological aspects, Lactobacillus
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