Mutation of the ptsG gene results in increased production of succinate in fermentation of glucose by Escherichia coli

Article Abstract:

Research indicates that increased production of succinate, an ester of succinic acid, results from a gene mutation occurring in glucose fermentation by Escherichia coli. The ptsG gene mutation, which emerged spontaneously, demonstrated reduced glucose phosphotransferase activity and an absence of normal glucose repression.

United States, Statistical Data Included, Gene mutations, Gene mutation, Genetic aspects, Fermentation

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Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli

Article Abstract:

Overexpression of phosphoenolpyruvate carboxylase is performed to enhance the fermentative production of succinic acid from glucose using Escherichia coli as biocatalyst. Experimental data show tha induction of carboxylase improves the concentration of succinic acid by as much as 350%, making it the major fermentation product by weight.

Analysis, Gene expression

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Production of succinic acid through overexpression of NAD+-dependent malic enzyme in an Escherichia coli mutant

Article Abstract:

The production of succinic acid through overexpression of NAD+-dependent malic enzyme in an Escherichia coli mutant was examined by overexpressing and purifying the enzyme to near homogeneity. Results reveal that when malic enzyme was placed under culture conditions in a strain of E. coli which was unable to ferment glucose, fermentative metabolism of glucose was restored. The major product of fermentation was, on the other hand, succinic acid.

NAD (Coenzyme), Malic acid, Nicotinamide adenine dinucleotide

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