Mycobacterium xenopi IS1395, a novel insertion sequence expanding the IS256 family

Article Abstract:

An insertion sequence (IS) of Mycobacterium xenopi, called IS1395, was isolated and sequenced. IS1395 has a 1323 bp element and is present in up to 18 copies in the M. xenopi genome and may be found in an M. xenopi extrachromosomal element. It encodes a putative transposase of 415 amino acids which exhibits sequence homology to the Staphylococcus aureus IS256 family. IS1395 represents a useful epidemiological tool for M. xenopi strain typing since it offers a variety of restriction fragment length polymorphism patterns.

Genetic aspects, Mycobacterium

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Recombinant BCG expressing the leishmania surface antigen Gp63 induces protective immunity against Leishmania major infection in BALB/C mice

Article Abstract:

The recombinant bacillus of Calmette and Guerin (BCG) vaccine effectively provides protection against Leishmania major in immunized BALB/c mice. The recombinant vaccine was developed by cloning and expressing the gp63 gene of Leishmania major in BCG under the influence of two microbial expression systems. The capability of recombinant BCG to decrease lesion development does not depend on the mode of vaccination. The experimental set up of the development of the recombinant BCG vaccine is discussed.

Care and treatment, Analysis, Innovations, Cloning, BCG vaccines, BCG, Leishmaniasis

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Impairment of sterol biosynthesis leads to phosphorus and calcium accumulation in Leishmania acidocalcisomes

Article Abstract:

Results show that inhibition of sterol biosynthesis induces the formation of acidocalcisomes which are shown to contain calcium, phosphorus and oxygen by electron spectroscopic imaging and spectroscopy.

Brazil, Research, Methods, Testing, Observations, Lysosomes, Electron spectroscopy, Enzyme inhibitors, Developmental cytology, Cell organelles, Organelles, Ketoconazole, Terbinafine

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