Neural tube and notochord promote in vitro myogenesis in single somite explants

Article Abstract:

Examination of the dependence of somite myogenesis on neural tube/notochord, using a newly developed in vitro assay, reveals that myogenesis in single somite explants is stimulated by neural tube and this stimulation requires close somite neural tube proximity. Myogenesis induced by neural tube does not require somite segmentation. Myogenic cell numbers in somites are increased by continuous exposure to neural tube. A different mechanism explains the promotion of in vitro myogenesis in single somite explants by notochord.

Analysis, Neural tube, Myogenesis, Somite, Somites (Embryology)

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Effects of the mesonephros and insulin-like growth factor 1 on chondrogenesis of limb explants

Article Abstract:

An experiment designed to test the effects of the mesonephros on limb development in vitro indicate that insulin-like growth factor-I (IGF-I) may be responsible for the early development of limb explants. Plant cultures treated with mesonophros exhibited faster growth and morphological differentiation and a well-organized cellular matrix than the untreated groups. Furthermore, the administration of an IGF-I antibody to the cultures removed this effect.

Growth, Extremities (Anatomy), Insulin-like growth factor 1, Insulin-like growth factor I, Cartilage

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WNT/beta-catenin pathway up-regulates Stat3 and converges on LIF to prevent differentiation of mouse embryonic stem cells

Article Abstract:

A study on pluripotency of embryonic stem cells based on wingless-type MMTV integration site family gene and beta catenin pathways is presented.

United States, Science & research, Cell proliferation, Embryonic stem cells

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