New conditions for generation of gynogenetic half-tetrad embryos in the zebrafish (Danio rerio)

Article Abstract:

Gynogenetic half-tetrad embryos of zebrafish, Danio rerio, can be generated using pressure between 6140 psi and 6840 psi. Viability is optimal when the pressure of 6140 psi and 6240 psi are used. The use of hydraulic pressure in this range interrupts the microtubule-mediated segregation during meiosis II. Generation of the expected ploidy, lack of paternal alleles, and demonstration of homozygous and heterozygous status for different markers are achieved using microsatellite markers. Triploid embryos are produced by early pressure treatment of eggs fertilized with nonirradiated sperms.

Methods, Usage, Fishes, Pressure, Pressure (Physics), Animal reproduction, Gynogenesis

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New isozyme loci in faba bean (Vicia faba L.): genetic analysis and mapping using trisomics

Article Abstract:

Segregation analysis has been used to identify the location of the locus Aat-2 on chromosome 3 of faba bean (Vicia faba L.). It was also possible to confirm previous research positioning both Pgd-p and Sod-I on chromosome 6. The latest research suggests that the locus Prx-1 could be linked with chromosomes 4 and 5, but Prx-1 was unambiguously located on chromosome 5 in previous work. Analysis of segregating isozyme loci did not reveal a single case of linkage relationship.

Faba bean

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Generation means analysis of climbing ability in common bean (Phaseolus vulgaris L.)

Article Abstract:

A study was conducted to determine the inheritance of climbing capacity traits in three crosses made within and between gene pools, using generation means analysis. Results showed the importance of additive compared with the dominant-additive portion of the genetic model. Generation means analysis and estimates of heritability suggested that the inheritance of plant height and internode length in climbing beans is relatively simple.

Colombia, Climbing plants, Common beans

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