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Occurrence of a sequence in marine cyanophages similar to that of T4 g20 and its application to PCR-based detection and quantification techniques

Article Abstract:

A study was conducted to characterize a set of polymerase chain reaction primers that identify sequences in DNA from cyanophages infecting phycoerythrin-containing marine Synechococcus strains. Cyanophage DNA was prepared from liquid lysates using phenol extraction and alcohol precipitation. Genetically distinct marine cyanophage isolates which lysed Synechococcus sp. strain WH7803 were determined by plaque assay.

Author: Fuller, Nicholas J., Wilson, William H., Mann, Nicholas H., Joint, Ian R.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
Research, DNA, Polymerase chain reaction

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Marine and freshwater cyanophages in a Laurentian Great Lake: Evidence from infectivity assays and molecular analyses of g20 genes

Article Abstract:

Populations of viruses that infect a marine cyanobacterium were discovered to be well distributed in the lake, during the process of characterization of viruses in one of the Laurentian Great Lakes (Lake Erie). Analyses of the gene encoding the g20 viral capsid assembly protein suggest that these viruses and amplicons from natural populations and the ballast of commercial ships are related to marine cynophages.

Author: Wilhelm, Steven W., Carberry, Matthew J., Eldridge, Melanie L., Poorvin, Leo, Saxton, Matthew A., Doblin; Martina A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Risk factors, Bacterial infections

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Diversity of microcystin-producing cyanobacteria in spatially isolated regions of Lake Erie

Article Abstract:

A study on the diversity of microcystin-producing cyanobacteria in the western basin of Lake Erie was conducted using sequence analysis of mcyA gene fragments. The study has identified a genetic element not associated with any known toxic Microcystis organism, suggesting the presence of earlier unknown sequence diversity within the mcyA genes of this genus.

Author: Wilhelm, Steven W., Rinta-Kanto, Johanna M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006

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Subjects list: Genetic aspects, Cyanobacteria, Environmental aspects, North America, Lake Erie
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