Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinatn bacteria

Article Abstract:

Researcher demonstrate that genetically engineered bacteria that normally colonize the roots of poplar trees can survive in the rhizosphere and degrade trichloroethylene. Trichloroethylene is a common groundwater pollutant at hazardous waste sites. Poplar trees grow quickly and a single five-year-old tree could process 53 gallons of polluted water per day.

Rhizobium, Microbial genetic engineering

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Stationary-phase quorum-sensing signals affect autoinducer-2 and gene expression in Escherichia coli

Article Abstract:

The presence and the effect of the stationary-phase quorum-sensing signals on the gene expression of early exponential-phase cells of auto-inducer-2-deficient strain Escherichia coli DH5(alpha) was examined using DNA microarray. Results suggest that an additional quorum-sensing system exists in Escherichia coli and the gene expression can be controlled as a network with different signals working at different growth stages.

Science & research, Analysis, Usage, Gene expression, DNA microarrays

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Oxidation of trichloroethylene, 1,1-dichloroethylene, and chloroform by toluene/omicron-xylene monooxygenase from Pseudomonas stutzeri OX1

Article Abstract:

The ability of toluene/o-xylene monooxygenase (ToMO) from Pseudomonas stutzeri OXI and expressed in Escherichia coli in degrading trichloroethylene (TCE), 1,1-dichloroethylene (1,1-DCE) and chloroform has been studied. The ToMO gene cluster was cloned downstream of the lac promoter in the isopropyl-beta-D-thiogalactopyranoside-inducible multicopy vector pGEM-3Z. Results indicate that stoichiometric amounts of chloride have been released, which indicates that mineralization has occurred. The measured values are 2.6, 1.5 and 2.3 Cl- atoms per molecule of TCE, 1,1-DCE and chloroform, respectively.

Environmental aspects, Microbiological synthesis, Pseudomonas, Chloroform, Trichloroethylene, Dichloroethylene, Vinylidene chloride

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