PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp

Article Abstract:

Primers consisting of parts of the fimA gene sequence are used for the polymerase chain reaction (PCR) amplification and detection of Salmonella strains. The fimA gene sequences, encoding the major fimbrial unit, are unique to Salmonella strains. The utilization of the fimA gene as a probe to differentiate between Salmonella and non-Salmonella strains provides a valuable diagnostic tool. The PCR method is effective in detecting Salmonella strains from contaminated samples of food and milk.

Methods, Identification and classification, Salmonella typhimurium

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Development and application of pathovar-specific monoclonal antibodies that recognize the lipopolysaccharide O antigen and the type IV fimbriae of Xanthomonas hyancinthi

Article Abstract:

Research was conducted to create a specific immunological diagnostic assay for yellow disease in hyacinths using monoclonal antibodies (MAbs). The MAbs were obtained by immunizing mice with ppurified fimbriae and shear fractions of X hyacinth. It was observed that antifimbria MAbs reacted with various fimbrial epitopes. The cross-reaction of the MAbs with all strains of X hyacinthi tested revealed that the Rha-Fuc O antigen is conserved within the species.

Endotoxins, Diseases and pests, Hyacinths, Antigen-antibody reactions

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Analysis of the type IV fimbrial-subunit gene fimA of Xanthomonas hyacinthi: application in PCR-mediated detection of yellow disease in hyacinths

Article Abstract:

A PCR-based technique for detecting Xanthomonas hyacinthi infection of hyacinths is described. This pathogen causes yellow disease of hyacinths and other members of the Liliaceae family.

Bacteria, Phytopathogenic, Phytopathogenic bacteria, Bacterial diseases of plants, Bacterial plant diseases

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