PCR-based identification of MAT-1 and MAT-2 in the Gibberella fujikuroi species complex

Article Abstract:

Results suggest that MAT allele sequences are useful in the screening of all sexually fertile strains of the Gibberella fujikuroi species for their mating type and phylogenetic relatedness to other species. Data indicate that the MAT sequence derived primer sequences do not amplify sequences from Fusarium species suggesting phylogenetic unrelatedness.

United States, Analysis, Usage, Polymerase chain reaction, Genetic markers, Phylogeny, Fusarium

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Simple sequence repeat markers distinguish among morphotypes of Sphaeropsis sapinea

Article Abstract:

Data is provided on four morphotypes of Sphaeropsis sapinea, a Pinus spp. fungal endophyte that causes symptomless infections. Polymerase chain reaction amplification and short-sequence-repeat primer pair development were used to determine the morphotypes; types A and C were closely related, while B was genetically diverse and I was identical to Botryosphaeria obtusa, another endophyte.

Statistical Data Included, Research, Plant genetics, Parasitic plants, Endophytes

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Transfection of Diaporthe perjuncta with Diaporthe RNA virus

Article Abstract:

The Diaporthe RNA virus transfected Diaporthe perjuncta exhibit different morphological characteristics than those isogenic virus-free strain in the production of yellow pigment, a decreased growth rate, and absence of sporulation. However, there is no difference in virulence between the two, suggesting that the virus can be developed into a biological control agent for Diaporthe canker.

Physiological aspects, Host-virus relationships, RNA virus infections, Transfection

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